Backup Failure with specific NIC-Interface

Hello,

Now we have a Backup Exec Media-Server with a additional 10Gbit-NIC-Interface.
Both NIC-Interfaces, the old and the new one, are on the same Subnet.
If we configure a backup-job with selecting the new 10G-NIC-Interface, the Backup Job results in a Failure.

Error Message: Can Connect to Agent

But Connection-Pings are succesfull.

Whats wrong?
It is possible to use the second 10Gbit-NIC-Interface on the same subnet for Backup or not ?

regards